We develop technologies for visualizing chromosomes in situ in order to study the 3D organization of the genome. One such technology is Oligopaints, an oligo-based approach for FISH originally developed in Ting Wu’s lab by Eric and Brian Beliveau. Oligopaints can be used to image single copy regions as small as 10 kb or less and as large as entire chromosomes. These probes are also compatible with super-resolution imaging, can be designed to distinguish individual alleles, and can work in a multiplexed fashion.
Our protocol for scaling Oligopaints to label entire chromosomes in fly (see image below), mouse, and human cells can be downloaded here