Studies of chromosome positioning have been stymied by the lack of affordable, high-resolution FISH probes, which are usually targeted to only a few loci at a time. Moreover, not only are conventional technologies not a practical source of probe for use in high-throughput methodologies, they fall short of revealing the location of whole chromosomes or specific sub-chromosomal regions in interphase nuclei. In contrast to conventional approaches, Oligopaints are computationally designed, synthesized on microarrays, and generated via PCR amplification. This strategy provides precise control over the sequences they target and allows for multicolor imaging of regions ranging from a few Kb to whole chromosomes with the same basic protocol. Our previous work generated Oligopaints to the entire unique portion of the Drosophila genome in a chromosome arm-specific manner (see banner above). More recently, we have also synthesized Oligopaints to three entire human chromosomes – chr18, 19, and 22 – in an effort to paint the entire the human genome (see below). These paints are renewable, cost-effective, and versatile as they can be amplified to specifically label different 3D chromatin features (TADs, compartments, chromatin states etc.). These expanded high-resolution chromosome paints allow us to better visualize epigenome organization across individual chromosomes and study the regulation of architectural proteins and their effects on nuclear, cellular, and physiological phenotypes.
If you need FISH probes, we are happy to design and generate custom Oligopaints for your experiments. We also have a growing repository of libraries against multiple species and genomic targets, which we have already tested and validated.
Contact Sonny for more information.