Oligopaints is an oligo-based approach for FISH co-developed by Eric Joyce and Brian Beliveau in Ting Wu’s lab. Oligopaints can be used to image single copy regions as small as 10kb or less and as large as entire chromosomes with the same basic protocol. These probes are also compatible with super-resolution imaging, can be designed to distinguish individual alleles, and can work in a multiplexed fashion for sequential genomic labeling.
Original manuscript describing development of Oligopaints:
*Beliveau BJ, *Joyce EF, Apostolopoulos N, Yilmaz F, Fonseka CY, McCole RB, Chang Y, Li JB, Senaratne TN, Williams BR, Rouillard JM, Wu CT. Versatile design and synthesis platform for visualizing genomes with Oligopaint FISH probes. PNAS. 2012 Dec 26. PMID: 23236188. *co-first authors
General information about Oligopaints can be found on the Oligopaints Website hosted by the Wu lab
An Oligopaints design platform developed by the Beliveau lab can be found at paintshop.io
Our protocol for scaling Oligopaints to label entire chromosomes in fly (banner image), mouse, and human cells can be downloaded here